In the Standard Plate Count method it is assumed that the bacterial cells are diluted to an end point where a single cell divides giving rise to a visible colony on a plate, although this assumption is not always correct.

answer: - few nutrients

high pressure

low temperatures

explanation: 1. few nutrients:   open-ocean zone is located way far from the land, which is the main source of the essential nutrients.2. high pressure: pressure increases by 1 atmosphere for every 10 meters increase in depth.3. ample sunlight: a large fraction of the sunlight is reflected back to the atmosphere from the sea surface. 4. varying salinity: below the thermocline, the water is isolated from the atmosphere so the salinity remains stable over the year.5. low temperatures:   the temperature of open-ocean zone ranges from a low of -2°c to an average of 17°c

there are so many examples for that in different areas, like biology experiment carried out in our lab recently.

here's one link:

you didn't give any options but heres what i can tell you: an inference is likely to change based on additional observations. the latter is something that you have seen or sensed using your sensory organs while the former is like a conclusion out from what you have observed.