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Biology, 13.03.2020 04:56 toxic12396green24
The secondary antibody is attached to an enzyme (HRP) that chemically changes the enzyme-substrate, turning it from a colorless solution to a blue solution. If you ran an antibody detection ELISA with positive control wells, negative control wells, and experimental wells, predict which wells of your experiment should turn blue, which should remain colorless, and which wells you are not sure about and why.
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The secondary antibody is attached to an enzyme (HRP) that chemically changes the enzyme-substrate,...
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