Suppose you perform a serial dilution of Amylase from a stock solution with a concentration of 500ug/mL. In five tests tubes you add 4mL of water. Then you aliquot 1mL of the stock solution into test tube 1 and vortex. From there you aliquot 1mL from test tube 1 into test tube 2. You continue to mix this way into each consecutive test tube. What is the final concentration in test-tube 3?

The final concentration in test-tube 3 is = 4ug/mL

Explanation:

Dilution factor = V initial / V final

Concentration of Stock solution = 500 ug/mL

5 test tubes are prepared with 4 mL of water

1 ml of stock solution is added to the 1st test tube.

therefore, the total volume of 1st test tube is = 4 ml + 1 ml = 5ml

∴ the concentration of 1st Test tube →

 C = m/V   [m= mass (solute) , v= volume]

     =500 ug / 5 ml  = 100 ug/ml

From 1st test tube, 1 ml is taken out and that is added to the 2nd  test tube.

∴ the concentration of 2nd Test tube = 100 ug / 5ml

                                                                = 20 ug/ml

From  2nd test tube, 1 ml is taken out and that is added to the 3rdd  test tube.

∴ the concentration of 3rd Test tube = 20 ug / 5ml

                                                              = 4 ug/ml

The final concentration in test-tube 3 is = 4ug/mL

what statement best describes the difference between the sympathetic and parasympathetic nervous systems?

the sympathetic nervous system prepares the body for intense physical activity and is often referred to as the fight-or-flight response. the parasympathetic nervous system has almost the exact opposite effect and relaxes the body and inhibits or slows many high energy functions.

it is because according to the process , scientific inquiry is a process with many steps because each step occurs to the scientist and if you just skip a step there would be no steps to scientific inquiry.