In the presence of a non-competitive inhibitor a Lineweaver-Burk plot has a higher y-intercept than a control (without any inhibitor), and the x intercept is the same as that for the control. Explain what this indicates.

The Lineweaver- Burk plot is representation of Lineweaver-Burk equation of enzyme kinetics in graphical plot that is presented by the Hans Lineweaver and Dean Burk.

In the presence of a non competitive inhibitor shows higher y-intercept than a control in absence of any inhibitor whereas the x intercept is the same as the control that suggests or tells that maximum enzyme activity of the reaction is decreased, Higher Y intercept than controls means that it is same as 1/Vmax. The X intercept indicates that there is no effect on Km of the enzyme reaction.

step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.