Biology, 25.03.2020 23:09 joynerjaila
Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads to which the polynucleotide 5'--3'
has been covalently attached. After a short incubation, the beads are then extracted from the mixture. When you analyse the cellular nucleic acids that have stuck to the beads, what will be most abundant?
mRNA because it is the only type of RNA that is polyadenylated and the poly (A) tail would be able to base pair with the strands of poly(T) on the beads and thus stick to them. DNA would not be found in the sample, as the poly (A) tail is not encoded int he DNA and long runs of T are rare in DNA.
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Total nucleic acids are extracted from a culture of yeast cells and are then mixed with resin beads...
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