You think you have nitrogen fixers in an anaerobic part of your Winogradsky column. You cap the column with 15N2 gas (a non-radioactive N isotope) and want to identify the active N2 fixers in this region of the column both metabolically and molecularly. Describe in detail the approach you would take to identify the active nitrogen fixers

The experiment will have to be carried out with three replications (biological replicates). Set up 3 identical columns that have been capped with gas and incubate for up to 24 hours. This will allow the nitrogen fixing bacteria time to carry out the process of nitrogen fixation. Collect 100 g of sample of soil from each of the column and perform the acetylene reduction assay, which measures nitrogenase activity.

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image result for how many calories of energy will 20 grams of carbohydrates provide in one gram provides 4 calories

consuming the right balance of protein, carbohydrates and fat can you maintain a healthy weight and optimize your energy levels. protein and carbohydrates both contain 4 calories per gram, while fat provides 9 calories per gram.