The process of alternate splicing of a primary mRNA transcript is shown above. What question can best be asked about this process?
A) How does DNA transcribe a molecule of RNA?
B) Why do prokaryotic genes contain non-coding regions?
C) What is the cause of mutations in the exon regions of DNA?
D) How can multiple polypeptides be produced from a single gene on the DNA?

The best question that can be asked from the given process of alternate splicing is that, How can multiple polypeptides be produced from a single gene on the DNA?

Option D

Explanation:

The alternate splicing is the process of splicing the gene code during the gene coding expression which results into a single gene coding, which later codes for multiple protein.

So, the best question which could arise from the given scenario is that how could a multiple protein or multiple poly peptides are produced out from a single gene on the DNA. And the answers lies within the process of alternate splicing which splices the gene creating variation in the gene code leading to multiuity to 20000 expressions.