In a biochemistry lab students carry out a SDS-PAGE gel experiment on a protein witha known molecular weight of 45 kDa. Unfortunately, each student group made a different mistake during the protocol. Consider each mistake and explain how the mistake would alter the results for that group.
A. The sample was not mixed with sample buffer prior to loading on the gel.
B. The sample was mixed with sample buffer that did not contain SDS prior to loading on the gel.
C. The sample was mixed with sample buffer that did not contain a reducing agent prior to loading on the gel.
D. The student failed to add the protein to the sample buffer.
E. The scientist did not include a molecular weight marker standard on the gel.
F. The students attached the anode of the power supply to the bottom of the gel and the cathode to the top of the gel.
G. The gel was stopped when the bromphenol blue was about 1cm from the top of the gel.
H. The gel was never placed into the stain.

c

explanation:

1. amino acid refers to the fundamental building block of proteins, comprising of an amino group and a carboxyl group.

2. catalyst refers to any substance, which increases the rate of chemical reaction without being used up in the reaction.

3. a complex molecule containing he genetic code is dna or deoxyribonucleic acid.

4. an enzyme refers to a molecule of protein, which is unique in accelerating up a particular chemical reaction in the biological systems.

5. nucleic acid refers to an sequence of sugars, phosphates, and nitrogenous bases, for example, dna and rna.

6. a protein refers to a large, composite organic molecule comprising of a huge number of amino acids associated together with the of peptide bonds.

7. rna refers to a nucleic acid that takes part in the production of proteins.

8. substrate refers to a compound, which takes part in a chemical reaction, and is chemically modified by the reaction.

bacteria could be both, harmful and to us. harmful bacteria could make us sick and probably even by getting the chances of getting a disease, but good bacteria fight it off with your immune system. there is always bacteria everywhere we go. we need to make sure to wash our hands after using the restroom, before eating, after eating, after petting an animal too. so you see, bacteria is everywhere and is harmful or . i hope this . : ) : ) : )