What was the purpose of adding methotrexate to the cells expressing a genetically-engineered Dihydrofolate Reductase enzyme in the mitochondrion-targeting experiment we discussed in lecture?

Methotrexate inhibits the synthesis of DNA, RNA, and protein synthesis of the cells in the experiment.

Explanation:

Methotrexate competitively inhibits the enzyme dihydrofolate reductase (DHFR) which is involved in the synthesis of tetrahydrofolate from the inactive dihydrofolate. The methotrexate blocks the enzyme by binding to its active site thereby preventing further reaction of the dihydrofolate reductase enzyme and dihydrofolate. Tetrahydrofolate is a useful material for the de novo synthesis of thymidine, a nucleoside. And without thymidine, DNA synthesis is hindered. Methotrexate therefore inhibits the synthesis of DNA, RNA, thymidylates and of course protein synthesis.

answer; hypnagogic sensations)

events in order:

1.       vishnu schist

2. zoroaster granite

3. bass limestone

4. hakatar   shale

5. igneous intrusion

6. shinumo quartzite

7. dox sandstone

8. tapeats limestone

9. bright angel shale

10. muav limestone

11. temple butte limestone

12. redwall limestone  

nonconformity

answer: the unkar group shows angular unconformity because the layers are turned at angle.

unconformity

answer: specifically the angular unconformity occurs between the cambrian and precambrian rocks. the dox sandstone, shinumo quartzite, hakatar shale, and bass limestone have been filled. an angular unconformity occurs between the cambrian and precambrian rocks. the dox sandstone, shinumo quartzite, hakatar shale, and bass limestone have been filled.

disconformity

answer: the disconformities aremarked irregular lines between the sedimentaryrocks layers.