Biology, 07.04.2020 22:54 drewje12

Describe a strategy using restriction enzymes and gel electrophoresis that would allow you identify that you have obtained the Recombinant DNA plasmid (containing YFG). Be specific indicating the techniques you would use and expected results.

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Answer from: DestyHilBan1183

Use a step wise process

Explanation:

In order to find the presence of the recombinant protein:

Culture the cells that you believe harbors the plasmid for the recombination protein (+) and also cells that do not (-). This is your control.Extract the plasmid from both strains.Observe the plasmid map and the area of your insert. Use this to select the restriction enzymes at the beginning and the end of the YFG gene. You can also use a restriction site inside the gene but not in the plasmid for better control. Perform the restriction enzyme digest on both (+) and (-) according to your protocol and separate using gel electrophoresis. Observe the separate bands that you see. In the (+) there should be a band that is the size of your insert and a larger band that is the size of your plasmid control band and in the (-) there should be a single band that is the size of the plasmid but non the size of the insert.

Answer from: Quest

c. earths continental crust is thicker than its lithosphere.

because, the lithosphere is the outermost layer of the earth/ 100 km thick and is defined by its machanical properties.the lithosphere contains oceanic and continental crust that varies in age and thickness across locations and geologic time. the lithosphere is the coolest layer of the earth in terms of temperature. the term lithosphere should not be confused with the use of "geosphere" which is used to indicate all of earth systems, including the atmosphere, hydrosphere and biosphere. the surface of the earth can be divided into the continental crust and the oceanic crust. the continental crust is made mostly of granite while the oceanic crust is made of basalt. the average thickness of the continental crust is 25 miles and the average thickness of the oceanic crust is 5 miles. three types of rock, igneous, sedimentary, and metamorphic, make up the surface of the earth. that's why 3 option is preferable.

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