Could someone help me with what I should put in the blank spots and outside the boxes?

According to theories of how life began, how did early organic molecules begin to separate from the outside world? a: specialized enzymes were required b: chains of amino acids created a barrier c: formation of microspheres or vesicles d: rna catalyzed the formation of membranes

What mistake did john needham make that caused him to conclude that spontaneous generation for microorganisms occurred? a. he re-contaminated his boiled broth solutions. b. he destroyed the vital force in the solutions. c. he did not boil his broth solutions, only warmed them. d. he failed to seal his flasks of boiled broth. e. he allowed his assistant to conduct the experiment which he did not monitor closely.

This is an active transport mechanism by which cells pump sodium and potassium ions against the concentration gradient