From an evolutionary perspective, what is the significance of the reproductive
isolation developing between two populations.
Speciation due to reproductive isolation results in extinction of the ancestral
species.
When two populations con not interbreed, the flow of genetic information
between them stops.
This is incorrect. Reproductive isolation over time results in speciation, which
changes the type of competition from intra-species to interspecies, not the
amount of competition between individuals or populations.
This is incorrect. Whether a mutation is favorable or unfavorable determines
whether it results in greater fitness, not the mutation rate.

Explanation:

Descriptions:  

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segmentation=endoskeleton=backbone=coelom=tissues

1. is geographic distribution

2 is increasing complexity

3 is transitional forms