What is the correct sequence of events leading to successful RNA splicing?
I. cut is made at 3' splice site
II. cut is made at 5' splice site
III. 5' end of intron attaches to branch point
IV. two exons are spliced together
V. lariat is formed
VI. intron is released

Explanation:

1. In RNA splicing the mRNA is first cut at the 5' splice site, present at the 5' side of mRNA

2. Then the intron folds back on itself and forms a lariat

3. And then the guanine nucleotide in the consensus sequence at the 5' splice site bonds with the adenine nucleotide  (A) at the branch point through a transesterification reaction . And both the 5' cleavage and lariat formation occur in a single step

4.  After that a cut is made at the 3' splice site that cause  free of the intron.

5. Then the  intron is released as in form of  a lariat.

6. And then finally the two exons are spliced together and splicing has done.

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the dna molecules have a negative charge because of the phosphate groups in their sugar-phosphate backbone, so they start moving through the matrix of the gel towards the positive pole. power is turned on and dna fragments migrate through gel (towards the positive electrode).

explanation: