An enzyme catalyzed reaction is run with the kcat=20/min and the Km=4uM. Use the enzyme concentration from question 1 above. A very strong inhibitor is added creating an alpha value of 10, which then reduces the Vo down to 240 nM/min. What was the substrate concentration? Give your answer as a 2 digit number in units of uM.

estuaries are the partially enclosed coastal bodies of brackish water formed at the mouth of sea where river meets an ocean. it is a transition zone between river and sea water so estuaries are influenced to both marine environment such as tides, waves and to riverine such as flow of fresh water and sediments. the mixing of sea and fresh water provide high level of nutrients, making estuaries a high product habitats.

many estuaries suffers degradation from a variety of human activities like erosion from deforestation, overgrazing, overfishing, drainage and eutrophication.

so from the above statement answers will be

1. false as estuaries are formed due to both brackish and freshwater.

2.true, due to high productivity estuaries are breeding ground for many marine species.

3. direct and indirect activities of man that contribute to pollution and deposition of sediments.

if, i suppose that a bee stings me swinging my hand away from a bee. then that if, i persist patient when a bee or wasp areas on your surface to investigate a scent or to take h2o and i secreting heavily, the bug ultimately will move of its personal agreement. if i don't need to set for it to disappear, lightly and gently wipe it off with a part of the paper.