Enolase is an enzyme that catalyzes one reaction in glycolysis in all organisms that carry out this process. The amino acid sequence of enolase is similar but not identical in the organisms. Researchers purified enolase from Saccharomyces cerevisiae, a single-celled eukaryotic yeast that grows best at 37°C , and from Chloroflexus aurantiacus, a bacterium that grows best at the much higher temperature of 55°C . The researchers compared the activity of purified enolase from the two organisms by measuring the rate of the reaction in the presence of varying concentrations of substrate and a constant amount of each enzyme at both 37°C and 55°C .

Enzymes are influenced by changes in pH. Enzymes are best dynamic at ideal pH. It is the most favorable pH value - where the compound is most dynamic. Above and beneath to this ideal pH influence enzyme action. Accordingly enzyme show catalytic action at ideal pH.  

At the point when enzymes are st low or high pH. Hydrogen particles interface with amino corrosive present at dynamic site. In this way changes their state of amino acids, their legitimate direction and influence enzyme movement.  

While estimating action of enolase . 2 phospho glyceraldehyde is a substrate for enolase included a response tube. After appropriate in hatching measure of item is estimated (PEP). Proportion of PEP to 2 phospho glyceraldehyde gives thought regarding enzyme action.  

For negative control, we will take a response tube, without having any enzyme enolase in it. Along these lines we will ready to preclude any change of 2 phospho glyceraldehyde to PEP without enzyme.  

All compound present in our body are adjusted to as per the earth where we are living. In this manner makes us effective in C. aurantiacus ideal temperature for enolase is 55 degrees. So this catalyst will consistently work best at 55 degrees than at 37 degree .