Given the following nucleotide sequences.
Seq 1
Seq 2
A) Determine the number of mutations between the 2 sequences.
B) Translate each sequence into one letter amino acid code.
C) For each site in the sequence, determine the synonymous and non-synonymous sites.
D) Sum those sites to get a total for total synonymous and non-synonymous sites.
E) Calculate dN and ds for the sequences and provide the dN/dS ratio
where: dN = # of non-synonymous substitutions/ # of non-synonymous sites
dS = # of synonymous substitutions/# of synonymous sites
F) What is your conclusion on what is happening with regards to selection given your calculated ratio at the locus represented by these?

step 1 ammonium can be absorbed by plants, but some is converted into nitrates which are better for plants to absorb. this is called nitrification. 2. step 2 the deer eats the plants and uses the nitrogen-containing proteins for cell growth. 3. step 3 bacteria in the soil conduct nitrogen fixation to convert nitrogen gas from the atmosphere into ammonium. 4. step 4 some of the nitrates are absorbed back into plants, but denitrification breaks down the nitrates into nitrogen gas released back into the atmosphere. 5. step 5 nitrogen is found in the urine, which gets broken down into ammonium through the process of ammonification. 6. step 6 plants absorb the ammonium and begin assimilation, the process to add the nitrogen to protein.

b. read the phase at the intersection of any given temperature and pressure.

step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.