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Biology, 21.04.2020 17:24 amorosoavap5cejz

You encounter a strain of E. coli that is unable to grow or divide using lactose as a carbon source. You analyze the chromosomal genotype of these bacteria, and determine it to be: lacI- lacP lacO lacZ- lacY lacA You want to introduce an F plasmid into this bacterium in order to that restore normal lac operon function: i. e. all three protein-coding genes will be repressed in the absence of lactose and be induced in the presence of lactose. What is the minimum set of genetic elements that you must include on your plasmid in order to achieve this result

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