You were recently hired by a biotech company to test out their new restriction enzyme. This enzyme recognizes the following sequence: GGATCC. It makes a cut between the two Gs. You have a linear test sample of DNA to try out this new product: 5'--3' Question 3 (2 points) How many cuts do you expect this enzyme to make on the above DNA? Please enter a number. Question 4 (1 point) How many fragments do you expect to see on your gel? Assume that the are all different sizes.

The enzyme will make 2 cuts producing three fragnments.

Explanation:

Some enzymes called endonucleases cleave the phosphodiester bonds that exist in the DNA. Some cleave with the aid of recognition sites and are called restriction endonucleases while some cleave non-specifically.

 5'--3'.

The enzyme recognizes the sequence: GGATCC. and makes cuts between the two GG.

In the sequence, there are 2 of GGATCC sequence sites. thus the enzyme will make 2 cuts producing three fragments.

5'-GATCAGCTGACTG GATCCGTCCTCAACGTCAG GATCCAGCTTCAAG-3'

the oxygenated blood then leaves the lungs through pulmonary veins, which return it to the left heart, completing the pulmonary cycle. this blood then enters the left atrium, which pumps it through the mitral valve into the left ventricle. from the left ventricle, the blood passes through the aortic valve to the aorta.

deoxygenated blood leaves through the right ventricle through the pulmonary artery. from the right atrium, the blood is pumped through the tricuspid valve (or right atrioventricular valve), into the right ventricle.

geologic time scale (for gradpoint) =)