An inactively of muscle will cause local temperature to decrease and will produce less CO₂. Cold temperature and higher pH cause the oxygen-hemoglobin saturation curve to shift reflecting that hemoglobin releases oxygen.

Cold temperature and higher pH cause the oxygen-hemoglobin saturation curve to shift high reflecting that hemoglobin releases more oxygen.

Explanation:

In higher pH i. e. neutral or alkaline and low body temperature, the hemoglobin molecule attach more oxygen to their active sites. When the temperature of the blood increases, the hemoglobin stops or reduces the binding of oxygen to their active sites and lower the saturation level.    Oxygen-hemoglobin saturation level refers to the amount of oxygen binds with the hemoglobin. This saturation decreases with increase in temperature and lower pH while saturation level increases when the temperature of the body is lower and pH is high.

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