Which of the following are true in regards to the use of double stranded break (DSB) mechanisms for genome editing? Select all that apply. Group of answer choices One of the main advantages of these systems is that it is possible to generate mutations in a sequence-independent manner. The addition of foreign DNA can lead to specific gene modifications. These mechanisms are specific for prokaryotes. Repair of breaks by NHEJ can lead to gene inactivation.

One of the main advantages of these systems is that it is possible to generate mutations in a sequence-independent manner.

The addition of foreign DNA can lead to specific gene modifications.

Explanation:

Double strand break mechanism for genome editing deals with the cellular machinery that repairs in a timely manner the break through the error prone non-homologous end-joining (NHEJ) error prone pathway that often results in insertions and deletions.

One of the main advantages of these systems is that it is possible to generate mutations in a sequence-independent manner.

The addition of foreign DNA can lead to specific gene modifications.

"1. it should be, it has been for about 20 years, and it's really not much different in the end than breeding animals to have particular traits. there are always concerns about genetically engineered animals/plants affecting the environment in unexpected negative ways, but i can't think of an example where the fears didn't turn out to be baseless.  

first, as you point out, it would be a great advance in pharmacology, to be able to tailor drugs and their doses to me. the pros involve better medical treatment. the cons involve privacy issues like insurance companies denying coverage to people with genetic risk factors. looking at dna markers wouldn't affect the frequency of genetic diseases, but it would allow detection of them earlier. and ok, actually i suppose that if people used this information to avoid having children with genetic diseases, some might decrease in frequency."