You discover a pattern of a 20 amino acid sequence at the C-terminus of over 50 proteins in an organism. You hypothesize that this amino acid sequence is a signal sequence for localization into a specific organelle or cell compartment, but you don't know which one. How might you use microscopy to investigate the location of proteins with this unique C-terminus sequence?

It depends on the type of microscope that you have  

Explanation:

For example, in the case that you have a fluorescence microscope, it is possible to create a fusion construct of the domain fused to the Green Fluorescence Protein (GFP) and then introduce it into the cell. The observation of fluorescence from this construct enables to confirm the existence of the signal sequence (i.e., the C-terminal domain) in the organelle

interphase   is the cell cycle stage in which the cell acquires nutrients, creates and uses proteins and other molecules and starts the process of cell division by replicating the dna. it is the longest stage in the eukaryote cell cycle.

the following   can go wrong during interphase:   a base pair could be mismatched during the duplication process causing a mutation.   correct answer: c

explanation:

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.