You have identified a novel gene, Ums1, whose protein product is a component of the RISC in the nematode C. elegans. You believe this protein (like all the RISC proteins) is critical for the production of miRNAs, in particular ones that slice the mRNA for a gene called Trtn1. Describe how you would use RNAi in worm lysates to knockdown Ums1 and show that slicing of Trtn1 is inhibited without Ums1. Include details like what part of Ums1 you would target with siRNAs, how trigger RNA would be delivered to the worms, and how you assay for sliced or unsliced Trtn1 mRNA. Show what positive results would look like (probably with an illustration of an autorad or bar graphs).

Me plz he did not infringe upon the policy making powers that he felt the constitution gave congress. but the determination of foreign policy became preponderantly a presidential concern. when the french revolution led to a major war between france and england, washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-british. rather, he insisted upon a neutral course until the united states could grow stronger. which provides the best objective summary of this excerpt? president washington made a good choice when he decided not to accept the recommendations of his advisors and instead insisted that the united states stay neutral during the war between france and england as president, washington believed that the united states should remain neutral in foreign policy even though he had advisors who recommended that he take sides during the war between france and england. washington did not make policy decisions during his presidency because he did not want to interfere with the policy-making powers that the constitution gave congress. washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-britis