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Without adding specific regulatory proteins, how might you stimulate transcription from the gene of interest relative to the transcription of the other genes on your DNA template? Add acomplementary to, that can bypass the first nucleotide incorporation step and permit efficient transcription at low nucleotide concentrations. For this experiment to work, the fourshould be provided atconcentrations. Addingconcentrations atconcentration will minimize initiation byat other sites.
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