A culture of insulin-responsive cells is analyzed after stimulation with insulin. The insulin receptors are then isolated (assume that during the isolation protocol the insulin is removed from the receptor). Half of the sample is analyzed by SDS-PAGE followed by Coomassie blue staining. The other half of the sample is analyzed via SDS-PAGE followed by a Western blot. The primary antibody used in the blot recognizes phosphorylated tyrosine residues. Predict how many bands will be seen on the SDS-PAGE compared with the Western blot and explain your answer.

The α and β subunits of the insulin receptor form two bands in the SDS-PAGE gel. Moreover, in the Western blot analysis, there is just one band corresponding to the β subunit, since this subunit contains phosphorylated tyrosines, while α subunit doesn't have.

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the liver is an organ endowed with a fantastic ability to regenerate

. thus, if a hepatectomy (ablation) of 70% of the hepatic mass is performed in the rat, there is complete recovery in 7 to 10 days.

prokaryotes are beings that have existed since the existence of life on earth, and do not come from another realm.

ribosomes are organelles that exist naturally in eukaryotes, either in free form or attached to the endoplasmic reticulum.

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the sphincter muscles at the end of the digestive tract is the anus.