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What was dr. willem johan kolff’s first step in using the scientific process to invent the hemodialysis machine? he built the machine and tried it with various patients, collecting data about its effectiveness. when a patient’s kidneys failed, he wondered if it would be possible to perform kidney functions with an external machine. he researched and collected data about numerous patients who exhibited symptoms of kidney failure. he drew conclusions from studies of various symptoms of kidney failure, and drew a design for the ideal external blood-processing machine.

To answer this question, researchers studied populations of the dusky salamander (desmognathus ochrophaeus) living on different mountain ranges in the southern appalachian mountains. the researchers tested the reproductive isolation of pairs of salamander populations by leaving one male and one female together and later checking the females for the presence of sperm. four mating combinations were tested for each pair of populations (a and b)—two within the same population (female a with male a and female b with male b) and two between populations (female a with male b and female b with male a). the proportion of successful matings for each mating combination was measured. for example, when all the matings of a particular combination were successful, the researchers gave it a value of 1; when none of the matings were successful, they gave it a value of 0. then the researchers calculated an index of reproductive isolation that ranged from 0 (no isolation) to 2 (full isolation). the reproductive isolation value for two populations is the sum of the proportion of successful matings of each type within populations (aa + bb) minus the sum of the proportion of successful matings of each type between populations (ab + ba). the table provides data for the geographic distances and reproductive isolation values for 27 pairs of dusky salamander populations.

Ayoung boy has been found and police are trying to locate his family they take a dna sample from him and begin collec dna samples from families who have missing children if police use dna samples only from the fathers, which type of dn technology can they use to identify the boy's parent? y-chromosome analysis omtdna (mitochondrial dna) analysis vntrs (variable tandem repeats) o pcr (polymerase chain reaction) analysis