How N-formylmethionine (fMet) is only associated with the 5' AUG initiation codon and not with internal AUG codons, given that methionine in both cases in encoded by an AUG in the mRNA.

Different transfer RNAs (tRNAs) selectively bind to N-terminal and internal AUG codons of bacterial polypeptides .

Explanation:

In prokaryotic organisms, N-formylmethionine (fMet) is coded by the start codon (AUG), thereby generating the most N-terminal residue of bacterial proteins. This residue (fMet) is a modified methionine amino acid that contains attached a formyl group to its amino group. Remarkably, it has been shown that formylmethionyl-tRNA only can bind to this initial AUG codon, while methionyl-tRNA binds to internal AUG codons.

c.) polymerase chain reaction

- "unlike the original dna fingerprinting method, dna profiling does not use restriction enzymes to cut the dna. instead it uses the polymerase chain reaction (pcr)? to produce many copies of specific str sequences. pcr is an automated procedure that generates lots of copies of a specific sequence of dna."

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