g One can measure ATP production from isolated mitochondria in vitro (in a test tube). Changing the pH of the solution that the mitochondria are in results in a similar change the pH of the intemembrane space. If the pH of the solution that the isolated mitochondria are in is raised and everything else remains constant, what is the effect on ATP synthesis

This will lead to an  decreases  in the ATPs synthesis. This is because low pH , high acidity favours ATP synthesis, because it increases the  proton concentration for electrochemical gradients needed for energy that  ATPase enzymes makes used of   synthesis  of ATPs.

Therefore a rise in the pH(low acidity) lowers protons levels, and therefore reduced electrochemical gradients , with a drop in energy for ATPs synthesis.

Explanation: