The picture shows a contractile vacuole of a unicellular freshwater organism. The contractile vacuole regulates the flow of water into and out of the cell in an aquatic environement. What condtions cause the contracile vacuole to fill with water? Your The concentration of water is greater outside the cell than inside the cell. The concentration of water inside the cell is the same as the concentration outside the cell. The temperature of water in the vacuole is higher than the temperature of its environment.