In the active site of the enzyme lotzase, a mutation of lysine 132 toaspartic acid causes a loss of lotzase function. Lotzase is most catalytically active at pH 7. Which is the following is the best reason why this might occur? a. a negative charge is needed for substrate binding.
b. a positive charge is needed for substrate binding.
c. a general acid is needed for catalysis to occur.
d. more than one of the following can be a good reason.

B. positive charge is needed for substrate binding

C. a general acid is needed for catalysis to occur

Explanation:

A mutation of lysine 132 toaspartic acid causes a loss of lotzase function as a result of the enzyme lotzase being most catalytically active at pH 7( neutral).

This condition is however achieved under conditions such as a positive charge being involved in the substrate binding and a general acid being involved in the catalytic process.

there are mainly three domains in biological classification namely; archaea, bacteria, and eukarya.

archaea includes all the prokaryotic cells which are characterized by their lipid membranes. they have branched hydrocarbon which is attached to glycerol through an ether linkage.

bacteria includes all the prokaryotic cells, the cell membrane of which are made up of the unbranched fatty acid chain. in addition, they are attached to glycerol through an ester bond.

eukarya includes all the eukaryotic cells, that is, the cells contain a nucleus and other membrane-bound cell organelles.

they can be autotrophic as well as heterotrophic, unicellular as well as multicellular

they include kingdoms like protista, plantae, animalia, fungi et cetera.

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explanation:

c

explanation: