You've discovered a gain-of-function mutant E. coli strain that makes red colonies on plates containing normal minimal medium; wild-type E. coli make white colonies. To identify the mutant gene causing this unuusal phenotype, you decide to use a plasmid library transformation approach. Which of the following do you need? A. A DNA construct in which sequences from the 5' end and sequences from the 3' end of the mutant gene flank a drug resistance gene.
B. Sequencing reagents, such as dNTPs, ddNTPs, and DNA polymerase.
C. A computer to blast the base pair sequence of the bacterial genomic insertagainst the genome sequence of your E. coli strain.
D. Cloned transposons from an organism other bacteria.
E. An E. coli genomic library made from the wild-type strain.
F. An E. coli genomic library made from the mutant strain.
G. Minimal media plates with no drugs or other supplements.
H. Minimal media plates containing the drug for which your genomic library plasmids have a resistance gene.
I. A sequencing primer that hybridizes adjacent to the insertion site of the bacterial genomic fragment in the library plasmids, and pointing in the 5'-to-3' direction towards the bacterial genomic DNA insert.

The correct answers are options

B. "Sequencing reagents, such as dNTPs, ddNTPs, and DNA polymerase".

C. "A computer to blast the base pair sequence of the bacterial genomic insert against the genome sequence of your E. coli strain".

F. "An E. coli genomic library made from the mutant strain".

I. "A sequencing primer that hybridizes adjacent to the insertion site of the bacterial genomic fragment in the library plasmids, and pointing in the 5'-to-3' direction towards the bacterial genomic DNA insert".

Explanation:

Plasmid library transformation approaches are very useful to characterize novel mutant bacterium strains. In order to perform a characterization study of this kind, several reagents and tools are needed, including:

- "Sequencing reagents, such as dNTPs, ddNTPs, and DNA polymerase". One important part of knowing what makes novel mutant bacterium different is to sequencing its DNA.

- "A computer to blast the base pair sequence of the bacterial genomic insert against the genome sequence of your E. coli strain". BLAST is a useful computer tool that compares and aligns DNA sequences, this allows to detect what are the nucleotides that are unique in the novel mutant bacterium.

- "An E. coli genomic library made from the mutant strain". Once DNA has been sequenced, an genomic library must be constructed to ensure that this novel mutant is preserved.

- "A sequencing primer that hybridizes adjacent to the insertion site of the bacterial genomic fragment in the library plasmids, and pointing in the 5'-to-3' direction towards the bacterial genomic DNA insert". Sequencing primers are needed when new genomic libraries are constructed. This short DNA sequences allow DNA polymerase to synthesize the DNA an replicate the genome of interest.

q: compare the parts of a cell and the cell as a whole to another common nonliving system (i.e., a car, a city,

a:

comparing this to the inside of a food building/company.

all cells are surrounded by a plasma membrane, which protects the cell from its environment, keeping toxic substances out of the cell. it regulates which molecules are allowed to enter and exit the cell.

regulates all cell activity such as cellular metabolism, and growth in addition to storing and maintaining the cell's dna for transcription and replication. determines what proteins to make.

contains the organelles; site of most cell activity

the place where ribosomes do their working activity.

builds the proteins.

prepares proteins for use or export.

responsible for breaking down and absorbing materials taken in by the cell.

maintains cell shape.

transforms one form of energy into another.

who knows

explanation: