You have isolated a mutant of Shigella dysenteriae bacteria that is resistant to kanamycin, and you want to find out what gene is responsible. Design a protocol using genetic selection to identify it

It is possible to identify the function of a gene by site-specific targeted mutagenesis (i.e., by using a reverse genetics approach).  

Explanation:

Targeted mutagenesis is an approach widely used in molecular biology in order to determine the functions of endogenous genes. Targeted mutagenesis is a type of genetic technique known as reverse genetics since it is a technology that enables to identify the function of a gene by modifying its product (i.e., its corresponding messenger RNA), thereby synthesizing non-functional proteins.

Moreover, it is also possible to identify the function of a gene by altering its expression, for example, by using small interfering RNAs (siRNAs) that inhibit protein translation through RNA interference (RNAi) mechanisms.

metamorphism is the change of minerals or geologic texture (distinct arrangement of minerals) in pre-existing rocks (protoliths), without the protolith melting into liquid magma (a solid-state change). the change occurs primarily due to heat, pressure, and the introduction of chemically active fluids. i hoped i !

not sure if this is what you'r looking for eubacteria and archaebacteria