Set up a mating between an Hfr strain and an F- (ade- his- bio-) strain and detect bio+ recombinant. Which of the following mating can let me select the recombinant by a single step (or a single selective medium)?
1. Hfr (ade + his+ bio+ Str-S) X F- (ade- his- bio-)
2. Hfr (ade+ his- bio-) X F- (ade- his- bio-)
3. Hfr (ade+ his+ bio+ Str-R) X F- (ade- his- bio- Str-S)
4. Hfr (ade+ his+ bio+ Str-S) X F- (ade- his- bio- Str-R)
Answers: 1
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Set up a mating between an Hfr strain and an F- (ade- his- bio-) strain and detect bio+ recombinant....
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