You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulin C-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolated from pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purified mRNA isolated from pancreatic α-cells from a patient during absorptive state. The third tube contains purified mRNA isolated from pancreatic β-cells from a patient during absorptive state. In not more than 2 pages (using 1.5 line space of Arial or Times New Roman fonts) provide answers for the following questions? 1) Which tube from the three is the most appropriate to use and why? 2) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide gene from the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 which has a 5¢-recognition site and a 3¢- HindIII in the MCS? 3) How can you guarantee a high expression of your protein in any expression vector?

as the gila monster consumes the eggs or young of the desert cottontail and gambel's quail, thus its main food supply is only accessible for a restricted time duration every year. however, for this, the gila monsters exhibit three adaptations, which elevate their survival rate in the desert condition.  

first, is that they possess huge bodies, and can consume greater concentrations of food during a small period. secondly, they require to feed infrequently as they can accumulate fat in their tails. ultimately, their rate of metabolism is very low, so that they can burn their reservoirs of fat gradually.  

rising temperatures because when water vapor in the air is cooled, it condenses. it turns back into a liquid and attaches to dust particles in the air. these very small water droplets group together to form clouds

if the hares very faster than they are now, and thus the lynx's hunting success rate drops because it will be harder to catch them, the rate that will change will be the pray-rate. the pray-rate will change because more hares will be able to survive, thus more individuals for reproduction, so the end result will be increasing population of hares.

the predator-rate will remain pretty much the same because there will be more than enough pray, but the hunting success rate is stagnant.