You are given 3 tubes of RNA and asked by your supervisor to clone the gene encoding the proinsulinC-peptide that will be used later to produce insulin. The first tube contains purified mRNA isolatedfrom pancreatic β-cells from a patient in a post-absorptive state. The second tube contains purifiedmRNA isolated from pancreatic α-cells from a patient during the absorptive state. The third tube containspurified mRNA isolated from pancreatic β-cells from a patient during the absorptive state. provide answers to the following questions?1) Which tube from the three is the most appropriate to use and why? 2) Describe the primary procedure (key steps no details) that you will follow to clone the C-peptide genefrom the RNA above into the only vector you have, a pUC expression vector cut open using EcoR1 whichhas a 5-recognition site and a 3- HindIII in the MCS? 3) How can you guarantee a high expression of your protein in any expression vector?

hey bro i looked it up on google the structure of the eye-spot in euglena viridie, e. ehrenbergii, and phacus pyriyorme is not as franzé haa described it. the so-called “crystal body” and “lens bodies” were not found in any of the individuals of the species examined by me. as earlier observers have stated, the eye-spot consists simply of a mass of pigment granules arranged in a single layer, and probably embedded in a protoplasmic framework, but the latter could not be seen, and its presence was only inferred from the fact that the pigment granules must be held together in some way, thd the eye-spot as a whole is capable of expansion, and that the pigment granules can be separated from each other.

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many phylogenetic trees have a single lineage at the base representing a common ancestor. a lineage that evolved early from the root and remains unbranched is called basal taxon. when two lineages stem from the same branch point, they are called sister taxa.

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explanation: