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1.Why did you use a pencil to mark the paper and not a pen? 2.Why do you have to use a different capillary tube for each sample? 3.Why is it very important in the amino acid chromatogram not to touch the paper with your fingers or hands? 4.If two samples have identical Rf values does this mean that they are necessarily identical molecules? Explain 5.What is your unknown? How do you know?
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