Explain why it is necessary to include only one chain-terminating/synthesis-termina ting nucleotide in each well of the electrophoresis instrument.

Explanation:

Generally for the formation of  phosphosdiester bond  between two nucleotide molecules  3'-OH group is needed between the  sugar molecule  of one nucleotide and the  OH group of the sugar present in the other nucleotide above it. However,in the single chain terminating molecule The 3'-OH is lacking.

Hence,DNA polymerace enzyme(which catalysis  DNA replication)  will not  be able to elongate the DNA molecules, through replications with the addition of di- (ddNTP). Thus inhibiting replication and therefore chain terminations,

answer; the tunica intima also called tunica interna, consists of elastic fibers,connective tissue and smooth muscle cells;

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