Determine the correct sequence of events during the creation of recombinant DNA. I. Plasmid is cut open with enzymes II. Target gene is cut out with enzymes III. Plasmid is inserted into the bacterium IV. Target gene is bonded to the plasmid with Ligase A. IV, III, I, II
B. II, I, IV, III
C. IV, I, II, III
D. I, II, III, IV

Why is the theory of evolution important? it disproves all other theories about how life began. it provides a topic for debate. it is a unifying concept in biology. it explains how life began. i know for sure, it is not "it is a unifying concept in biology."

African penguins, which inhabit the coasts of southern africa, were classified as an endangered species in 2010. two significant threats to their survival are ecosystem damage from oil spills and overfishing by humans. overfishing depletes the food supply of african penguins. the best method to reduce the threat of overfishing would be to . the risk of oil spills could be reduced by increasing the use of , which should oil consumption. if an oil spill does occur, could be used to remove the oil so the ecosystem may more quickly recover.

Genetic disorders can result when sister chromatids fail to seperate properly. during what phase is this problem most likely to occur?