Shyam being very inquisitive, didn’t listen to the teacher’s instruction properly. He took the thin layer of onion peel with a drop of water on the glass slide. He placed the coverslip on it and start observing the slide under a microscope. But he was unable to see the cells of onion peel properly. • What do you think he might have forgotten?
• What is the function of a dye?
• What values do you get from the above incidence?

he forgot to adjust the brightness and the curtain. Slowly move the knob so that the objective goes up. Continue until the image is okay

Pretty clear focus. Then use the knob, if available, for a clearer picture. If you are on a microscope with a moving specimen carrier, turn the coarse knob so that the table carrying the specimen moves down or away from the objective. This is exactly how to use a microscope focus regulator.

Explanation: