Biology, 21.09.2020 02:01 bekahmiller2001
You are trying to determine the sequence of a small protein. Acid hydrolysis has given an amino acid composition of G4T3P2V2A2Y2Q2D1S1K1M1N1R1K1. Reaction with FDNB gave an abduct with Glycine. Reaction with cyanogen bromide gave two peptides, a 5aa peptide with the sequence GGPVM, and a 19aa peptide with N-terminal sequence of ATYEQ. Proteolysis yielded the following results:
Protease Length N-term
Chymotrypsin 9 EQDGG
8 GGPVM
7 APKNT
V8 Protease 13 GGTSR
2 QD
9 GGPVM
Trypsin 4 YAPK
16 GGPVM
4 NTVQ
Required:
What is the sequence of the protein?
Answers: 3
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You are trying to determine the sequence of a small protein. Acid hydrolysis has given an amino acid...
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