subject
Biology, 21.09.2020 15:01 chjam265

Just when the Novel Prize looms clearly on the horizon, tragedy strikes: the reaction ceases to work well. The only difference between when the reaction was working and now is that the DNA polymerase is from a different source (isolated from a different organism). [NOTE: enzymes like polymerases used in most laboratories are purchased in kits from companies.] The Km of the "old" polymerase was 20 µM nucleotide, and the Km of the "new" polymerase is 100 µM. What is the first thing you would do to change the reaction conditions (using the new polymerase) in your attempt to get the reaction to work?

ansver
Answers: 1

Another question on Biology

question
Biology, 22.06.2019 00:30
Compare and contrast cladistics and phylogeny. what are their advantages and disadvantages? which is the most useful in understanding how an organism developed into its current form? which concept most strongly supports your opinion about the origin of any given organism?
Answers: 1
question
Biology, 22.06.2019 02:00
The phylogenetic tree illustrates the relationship between humans and our closest living relatives. the tree was based on biochemical comparisons, including dna and amino acid sequences. according to the biomolecular data, we could infer that a) the four organisms do not have a common ancestor. b) humans are more closely related to chimps than any other apes. c) chimps are more closely related to gorillas than they are to humans. eliminate d) there is no evidence if any relationship between the four branches on the tree.
Answers: 3
question
Biology, 22.06.2019 02:30
Ecologist have observed a sharp increase of sulfur dioxide in the air . what might be a possible consequence of excessive sulfur dioxide in the air ? a. acid rain b. ozone layer depletion c.ozone layer hole d.increase in sea levels
Answers: 1
question
Biology, 22.06.2019 08:00
What is the ecological role of food chains and food webs
Answers: 3
You know the right answer?
Just when the Novel Prize looms clearly on the horizon, tragedy strikes: the reaction ceases to work...
Questions
question
Mathematics, 25.01.2021 17:20
question
Mathematics, 25.01.2021 17:20
question
Mathematics, 25.01.2021 17:30
Questions on the website: 13722361