Which option is a limitation in an experimental design? O A. Having only one responding variable
O B. Having only one manipulated variable
C. Having multiple groups of test subjects
O D. Having a small sample size of test subjects

the answer is d

Explanation:

step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.