Answer the questions below using the graph 1. Describe the relationship between substrate and the product. What happens when you increase the amount of substrate? Predict what would happen if you used 20.g of substrate and explain your reasoning behind the prediction.

2. Explain why the maximum initial reaction rate cannot be reached at low substrate concentrations.

3. What is the optimum pH level for this enzyme? How do you know? Make a prediction about what you think the reaction rate would look like a pH = 2.

4. Enzymes function most efficiently at the temperature of a typical cell, which is 37 degrees Celsius. Increases or decreases in temperature can significantly lower the reaction rate. What does this suggest about the importance of temperature-regulating mechanisms in organisms? Explain