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Biology, 12.10.2020 19:01 nathanwhite2000
A common method for analyzing microtubule motor activity is to attach them to a glass coverslip (the tails stick very well to glass) and then incubate with microtubules, which will settle on them and then "glide" across the surface of the coverslip as the heads of the motors propel them. This is easily observable in the light microscope. In an assay such as this, how can one determine the directionality of the movement of the microtubules (plus- or minus-ended)? Assume that you do not know if the motor is a plus- or minus-end motor prior to the experiment.
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A common method for analyzing microtubule motor activity is to attach them to a glass coverslip (the...
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