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Biology, 17.10.2020 19:01 amy290

You are performing an experiment with Primase, using a specific DNA template. In this experiment, you provide NTPs (ATP, UTP, CTP, GTP) which contained only one radioactive phosphate group, in the alpha-phosphate position (the alpha- phosphate is the one attached to the sugar. the other phosphates are beta and gamma). After RNA synthesis occurs, you separate the resultant RNA strand any of the NTPs that have not been incorporated into the RNA, and measure the radioactivity of the newly synthesized RNA. A) In this experiment, would you expect the newly synthesized RNA be radioactive? Explain why. B) If you forgot to add CTP when you did the experiment, would the resulting RNA be radioactive?C) If you did the experiment, with all the NTPs provided, but only the beta phosphate was radioactive for each of the NTPs, would radioactivity be found in the RNA? Explain why. D) If you did the experiment, with all the required NTPs, but only the ATP was radiolabelled Would radioactivity be found in the RNA? Explain why.

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