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Biology, 21.10.2020 17:01 alopez117

One primer attaches to the __ end of the gene region on the 5' to 3' strand, another to the ___ end of the gene region of the 3' to 5' strand. This was maybe not explicit in the video. 3โ€™ and 5โ€™ refer to the orientation of the DNA strand. The 3โ€™ end is the end that has the OH group used for adding new nucleotides. The 5โ€™ end is the end where a phosphate sticks out from the ribose. The two strands that make up the DNA molecule run anti parallel to each other. Synthesis is always in the 5โ€™ to 3โ€™ direction (because that is where new nucleotides attach), and the template strand is read 3โ€™ to 5โ€™ (because it runs antiparallel).

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One primer attaches to the __ end of the gene region on the 5' to 3' strand, another to the ___ end...
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