Calculate the volume of plasmid and insert needed for a ligation reaction using the specifications for NEBL T4 DNA ligase (0.020 pmol vector, 0.060 pmol insert). This reaction is ligating a 3kb vector (25ng/μL) and a 2kb (10ng/μL) insert together, and the fragments have complementary sticky ends. Round final answers to the nearest hundredths place.

Bisphenol a (often called bpa) is a chemical found in products that people use every day, from water bottles to food containers to children's toys. unfortunately, bpa leaches out of its many products and makes its way into our bodies. what are the health effects of bpa exposure? ongoing research is finding that elevated exposure to bpa can affect a wide variety of developmental and physiological processes, but one of the first studies of bpa's health effects came about because of a simple mistake in the lab. at a laboratory at case western reserve university in 1998, geneticist patricia hunt was making a routine check of her female lab mice. as she extracted and examined developing eggs from the ovaries, she began to wonder what had gone wrong. she noticed that many of the eggs showed problems with their chromosomes, and some had irregular amounts of genetic material, which can lead to miscarriages and birth defects in mammals. she learned that a lab assistant had mistakenly washed the plastic mouse cages and water bottles with a harsh soap, releasing bpa from the plastic. knowing that bpa is an endocrine disruptor, a chemical that can enter organisms and mimic hormones, hunt set out to discover whether it had adversely affected her mice.

Suppose you have monohybrid pea plants in your garden and find that they produce round seed to wrinkled seeds in the ratio of 3: 1. if the allele are designated (r & r) receptively. what is the probable genotypes of the round seeds which produced f 1 ? rr & rr rr only rr & rr rr only rr only

What organ is the first to receive nutrients that have been absorbed from the digestive tract?