Your lab uses the annelid worm Lumbriculus variegatus as a model organism to study regeneration. Your studies would be helped by knowing which genes are present in this organism, but its genome has not been sequenced. A recent technique for gene determination is RNA-Seq, in which RNAs are directly isolated and sequenced. What is a disadvantage of using RNA-Seq for gene identification? a. RNA-Seq may miss genes transcribed in a very tissue- and temporal-limited fashion.
b. RNA-Seq relies on genes being homologous across species, but not all genes have homologs.
c. RNA-Seq works only in mammalian cells and therefore cannot be used with model organisms such as Lumbriculus or Drosophila.
d. RNA-Seq may selectively miss noncoding RNAs as compared to mRNAs.

a. RNA-Seq may miss genes transcribed in a very tissue- and temporal-limited fashion.

Explanation:

RNA-Seq is a methodology used in molecular biology to identify RNA transcripts by using deep-sequencing techniques. RNA-Seq has shown that the genome is often transcribed in the form of non-coding RNA sequences such as, for example, long non-coding RNAs (lncRNAs), microRNAs (miRNAs), etc., thereby redefining our vision about transcription in eukaryotic organisms. This methodology is based on the conversion of RNAs to cloned cDNA (complementary DNA) fragments that are further sequenced to be mapped against the reference genome. In consequence, tissue-specific-transcripts are not detected by RNA-Seq in cells in which these sequences are not transcribed.

why many students visually perceive stem as a vapid class?

for many students, however, stem subjects are optically discerned as vapid, tedious, and destitute of authentic-world applications. science and math have always been a major part of the high school curriculum, but many students have disengaged from these subjects because they don’t optically discern that they have any pertinence to the authentic world or students’ daily lives. for them, math and science have become a set of rules, procedures, laws, theorems, and formulas to memorize. some students even develop math apprehensiveness and cerebrate they are incapable of learning higher-level mathematics.

why is stem paramount?

as the u.s. economy changes, the ordinant dictation for stem-capable workers is growing. in may of 2014, the u.s. bureau of labor statistics counted more than 8.3 million stem jobs, which accounted for 6.2 percent of total employment in the cumulated states. that number is expected to perpetuate to increment as u.s. companies grow and innovate to compete in the ecumenical economy. according to the u.s. department of labor, the average salary for all stem jobs is $85,570, which is virtually double the average salary for all jobs.

many people still envision stem professionals as wearing a white lab coat and conducting research in a laboratory. in authenticity, some of the most in-demand stem fields are petroleum engineering, process engineering, and computer engineering. these fields that are experiencing shortages of qualified candidates are additionally among the highest paying, with the average petroleum engineer earning an annual salary of $147,520 in may 2014 according to the u.s. bureau of labor statistics.

in additament, stem subjects edify students valuable skills that are vital for prosperity in all vocations, even those that do not directly require stem erudition. engagement in stem subjects avails students develop quandary-solving, critical mentally conceiving, and leadership skills. these skills are so ecumenical, in fact, that statistics relinquished in the summer of 2013 by the u.s. census bureau designated that about three-quarters of the people with a bachelor’s degree in stem fields are not working in those vocations. many non-stem employers are poaching stem-qualified job candidates because they are more ingenious and collaborative than their non-stem counterparts.

as the ecumenical economy perpetuates to evolve, the desideratum for stem-capable workers will perpetuate to grow. by getting edifiers and students involved in the stem kineticism, an incipient generation of adroit workers will be poised to energize and expand this exhilarating field.

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what do the different brain waves do?

explanation:

brainwaves are produced by synchronised electrical pulses from masses of neurons communicating with each other. brainwaves are detected using sensors placed on the scalp. brainwave speed is measured in hertz (cycles per second) and they are divided into bands delineating slow, moderate, and fast waves.