A certain membrane protein is found on the ER membrane with the N-terminus on the ER side of the membrane and the C-terminus on the cytosol side of the membrane. The protein contains three transmembrane domains. What would be the orientation of the protein across the ER membrane if the gene were mutated such that the third transmembrane domain coded for hydrophilic amino acids

americans began to notice growing differences between the northern and southern sections of the country. there were more people living in cities in the north, which made it more urban. more people lived on farms in the south, which made it more rural. while many people were farmers in the northern states, there were now other ways to make a living, such as working in mechanical cotton gins factories manufacturing cloth, tools and other goods. people in the north supported high tariffs on goods imported from europe because the extra cost encouraged people to “buy american” instead. in the southern states, planters did not like tariffs because they sold most of their production to europe and bought their tools from there as well. southerners believed the protective tariff was something that only northern factory owners keep their profits high. so the southern politicians came up with an idea. they would opt out. they would simply ignore the protective tariff. it was ‘null and void’ in south carolina. the state would not collect the taxes on goods imported into south carolina, no matter what the president said they had to do. this decision led to the nullification crisis.

sheep is a herbivore animal whereas grasshopper belongs to the class chordates but both of them feed on greens.  

if grasshopper and sheep feeding on a pasture land, i do not think both of them will have any impact on each other. however, the number of the species can make difference. since sheep feed on greens, there is a possibility there left no pasture land for the grasshoppers or they may get extinct.

photosynthesis makes the glucose that is used in cellular respiration to make atp. the glucose is then turned back into carbon dioxide, which is used in photosynthesis. while water is broken down to form oxygen during photosynthesis, in cellular respiration oxygen is combined with hydrogen to form water.