By the end of Like Water for Chocolate, to what extent have characters been liberated? Specifically, Tita and Gertrudis

The final consummation of the passion between Tita and Pedro is both tragic and triumphant in that the light of Tita's inner fire is finally free to blaze, but only at the expense of her earthly life.

the correct answer is:

a population changes in response to a change in the environment.

answer:

sequence 1- deletion

                  2- duplication

                  3- insertion

                  4- substitute

                  5- inversion