One type of mutation that can give rise to cleidocranial dysplasia in humans is an STR that causes an increased number of repeated Glutamine (Q, Glu) amino acids in the Runx2 protein that is produced by the RUNX2 gene. Below is a portion of the coding DNA strand for RUNX2, along with the amino acid sequence encoded. There is an STR triplet (CAG) repeated 22 times in the "wild type" allele, which results in a repeated sequence of Q amino acids. Your mission is to design a diagnostic assay for determining the approximate number of CAGs. 5' â GTG GCT GCG CAA (CAG) 22 GAG GCG GCG GCG â 3' N-term. VAAQQ22EAAA C-term. a. First, you need to design a PCR reaction to amplify just this portion of the gene from the two homologous chromosomes (the gene resides on Chromosome 6). What would be appropriate primer sequences to use for this PCR reaction? Forward primer: 5'- Reverse primer: 5'- b. Once you have amplified the DNA, what are the next steps you need to perform in order to visualize the PCR products? Make sure you mention the "controls" that must be included in your experiment to provide a meaningful interpretation of the results. Please use no more than 3 simple sentences! 2 NAME: c. Draw and/or describe (in THREE or fewer sentences) your predicted results for at least three types of cases, assuming that up to 22 CAGs causes wild-type phenotype, 4 additional CAGs over the normal 22 is predicted to cause mild CCD (even in heterozygotes), and more than this is associated with severe CCD (even in heterozygotes)?